Figure 1

Representative region of the suppression subtractive hybridization (SSH) microarray of the Met and Db tumor lines. (a) The SSH libraries were arrayed, probed with fluorescence-labeled aliquots of each library, and scanned with a two-color fluorescent scanner. Green represents higher expression in the Met line, and red represents higher expression in the Db line. (b) Analysis of the same section of the SSH array with the GenePie function of ImaGene (BioDiscovery, Inc., Marina Del Rey, CA, USA) shows the relative percentage of signal intensity at each spot of the two fluorescent labels. Probes were selected for sequencing on the basis of high expression and differential expression. Osteopontin (OPN) was identified as being highly differentially represented in the Met subtraction library and is marked with arrows on the array in (a) and (b).