Transient expression of FGFR2 clones isolated from SUM-52PE. (a)Whole-cell lysates from 293 cells that had been transiently transfected withindividual FGFR2 clones were immunoblotted with an anti-FGFR2 antibody (lanes2-5). Whole-cell lysate from the SUM-52PE breast cancer cell line was loaded inlane 1 as a positive control, whereas whole-cell lysates from a mocktransfection of 293 cells was loaded in lane six as a negative control. (b)Whole-cell lysates from 293 cells that were transiently transfected withindividual FGFR2-C3 clones containing a Flag sequence were immunoblotted withan anti-Flag antibody (lanes 1, 2, and 4). Whole-cell lysates from a mocktransfection of 293 cells were loaded in lane 3 as a negative control.