Skip to main content
Figure 5 | Breast Cancer Research

Figure 5

From: Type V collagen induces apoptosis of 8701-BC breast cancer cells and enhances m-calpain expression

Figure 5

A) PCR amplification of the 512 bp product of L-mC cDNA (1), and diagnostic restriction analysis of the same yielding the expected 316 and 196 bp fragments after digestion with PstI endonuclease (2). M) Size marker PBR322 plasmid/HinfI. B) "Low cycle number"-PCR amplification of cDNA preparations from 8701-BC cells grown onto either type IV or type V collagen substrates in the presence of primers specific for L-mC and GAPDH cDNA fragments. PAGE 6%, SYBR Green I stain.

Back to article page