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Table 1 Primers used in representational difference analysis (RDA) and different PCR screening experiments

From: A candidate metastasis-associated DNA marker for ductal mammary carcinoma

Primer/product size

Primer sequence (forward)

Primer sequence (reverse)

RDA representation

R24, 5'-agcactctccagcctctcaccgca-3'

R12, 5'-gatctgcggtga-3'

RDA first-round hybridization

J24, 5'-accgacgtcgactatccatgaaca-3'

J12, 5'-gatctgttcatg-3'

RDA second-round hybridization

N24, 5'-aggcaactgtgctatccgagggaa-3'

N12, 5'-gatcttccctcg-3'

RDA third-round hybridization

Same as first round (J24)

Same as first round (J12)

PTEN (556 bp)

5'-ctcagattgccttataatagtc-3'

5'-tcatgttactgctacgtaaac-3'

MADS-IX (180 bp)

5'-aggtaggtagagtaacaggtttgttt-3'

5'-gatctgttctcccttttctttagctt-3'

FISH fragment (2 kb)

5'-actatgtatgtgagcatctgtttgc-3'

5'-tgcactcttcacttgagtaaacttg-3'

β-Actin (295 bp)

5'-tcacccacactgtgcccatctacga-3'

5'-cgacgtagcacagcttctcctta-3'

  1. FISH, fluorescence in situ hybridization.