Figure 1From: Inhibition of E2-induced expression of BRCA1 by persistent organochlorinesPhosphorImager images of hybridizations with BRCA1 (7.8 kb), pS2 (500 bp), and GADPH (1.3 kb) probes against the same northern blot membrane with 20 μg/lane total RNA from MCF-7. Concentrations of the chemicals are as shown in Fig. 2. The filter represents only one experiment. The collective data are presented in Figs 2 and 3. After hybridization with BRCA1, the filter was stripped and rehybridized with pS2 and then with GADPH. MDA-MB-231 data are not shown. E2, 17β-estradiol; Tox.Tech., toxaphene (technical mixture); Ici, ICI 182780; TCDD, 2,3,7,8 tetrachlorodibenzo-p-dioxin; PCB, polychlorinated biphenyl; EtOH, ethanol; DMSO, dimethyl sulfoxide.Back to article page