Figure 2
From: Novel mutations in the BRCA1 and BRCA2genes in Iranian women with early-onset breast cancer
SDS-PAGE analysis of the whole of exon 11 of BRCA2, revealing the capacity of the PTT technique to detect mutations within 4959 bp coding sequences in a single reaction with the use of a coupled transcription-translation system, TNT® T7 Quick for PCR DNA kit from Promega. Lanes 1 and 2 show the normal pattern of the full-length (181 kDa) protein from exon 11 of BRCA2; lanes 3–5 show three different sizes of truncated protein, which were identified on 6% SDS-PAGE. Arrows show the sizes and positions of the normal and truncated proteins. Lane 4 shows a mutation occurring close to the 3' end of exon 11 and producing a large protein for which the band migrated close to the top of the gel.