Skip to main content
Figure 1 | Breast Cancer Research

Figure 1

From: Caretaker Brca1: keeping the genome in the straight and narrow

Figure 1

Schematic representations of the (A) pim1-Q and (B) Rb-O targeting vectors. (A) Gene targeting strategy at the pim1 locus. Expression of the promotorless hygromycin resistance relies on the endogenous pim1 promotor once it is integrated by homologous recombination. Transfected cells are selected in hygromycin. (B) Gene targeting strategy at the Rb locus. The Rb-O contains a hygromycin gene expressed from a pgk promotor. Therefore, hygromycin resistance is exhibited regardless of the site of integration

Back to article page