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Figure 5 | Breast Cancer Research

Figure 5

From: P2Y2receptor activation by nucleotides released from highly metastatic breast cancer cells increases tumor growth and invasion via crosstalk with endothelial cells

Figure 5

ATP or uridine 5′-triphosphate (UTP) induced the phosphorylation of vascular endothelial (VE)-cadherin at tyrosine residue Y658 in endothelial cells (ECs). (A) ECs were treated with ATP or UTP (10 μM) for different periods of time (5 to approximately 360 minutes). Y658-phosphorylated VE-cadherin (88 KDa), VE-cadherin (130 KDa) and β-actin protein expression levels were determined by western blotting. (B) ECs were transfected with control- or P2Y2R-siRNA, and the ECs were treated with ATP or UTP (10 μM) for 30 minutes. Protein expression levels were determined as described previously. Significance compared to the control, **P <0.01; significance compared to ATP or UTP, #P <0.05; ##P <0.01.

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Breast Cancer Research

ISSN: 1465-542X

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