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Figure 1 | Breast Cancer Research

Figure 1

From: P2Y2receptor activation by nucleotides released from highly metastatic breast cancer cells increases tumor growth and invasion via crosstalk with endothelial cells

Figure 1

ATP release and P2Y 2 R expression and activity in various cell types. (A) The amount of ATP released into the extracellular medium was measured as described in Methods. Significance compared to MDA-MB-231, **P <0.01. (B) Total RNA was collected from endothelial cells (ECs), MCF10A, MCF-7 and MDA-MB-231, and P2Y2R (200 bp) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (125 bp) mRNA expression was analyzed by RT-PCR. The results were confirmed by at least two independent experiments. (C) Intracellular Ca2+ levels were determined in MDA-MB-231 and MCF-7 to measure the P2Y2R activity. Arrows indicate the points at which ATP or uridine 5′-triphosphate (UTP) (10 μM) was added. The net change in Ca2+ levels was normalized to (Fmax-F0)/F0. Significance compared to ATP or UTP, **P <0.01.

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