Skip to main content
Figure 3 | Breast Cancer Research

Figure 3

From: Nonhypoxic regulation and role of hypoxia-inducible factor 1 in aromatase inhibitor resistant breast cancer

Figure 3

Regulation of HIF-1α protein in LTLTCa cells. A) LTLTCa cells were treated with either vehicle, 1 μM lapatinib, 20 μM MAPK pathway inhibitor U0126, 20 μM LY294002 PI3K pathway inhibitor, 500 μg/ml trastuzumab or 100 nM RAD001 for 24 hours. Total protein was extracted and HER2 (P = 0.128), phospho- and total-ERK1/2 (P <0.0001 for p-ERK), phospho- and total-Akt (P <0.0001 for p-Akt), phospho- and total mTOR (P = 0.0071), phospho- and total p70 S6 kinase (P <0.0001), ERα (P <0.0001), HIF-1α (P = 0.0003), and β-actin were analyzed by Western blot. Shown are representative blots and overall densitometry results of n = 6 independent cell samples/group. Densitometry results are expressed as mean fold-change in protein levels compared to vehicle-treated cells after normalization to β-actin (mean ± SD, n = 6 independent cell samples/group; *versus vehicle, P <0.05; † versus vehicle, P <0.001, one-way ANOVA). ANOVA, analysis of variance; ERα, estrogen receptor alpha; HER2, human epidermal growth factor receptor 2; HIF-1α, hypoxia inducible factor 1 α subunit; n, number; SD, standard deviation; mTOR, mammalian target of rapamycin.

Back to article page