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Figure 5 | Breast Cancer Research

Figure 5

From: Role of the androgen receptor in breast cancer and preclinical analysis of enzalutamide

Figure 5

Enzalutamide inhibits estradiol-mediated proliferation of breast cancer cells, while bicalutamide does not. MTS proliferation assays were performed on (A) MCF7s cells and (B) BCK4 cells treated with vehicle control, 10 nM estradiol (E2), 10 μM enzalutamide (Enza) or a combination of E2 and Enza. Error bars represent standard error of the mean (SEM). **P < 0.01, ***P < 0.001 for E2 versus E2 + Enza, analysis of variance (ANOVA) with Bonferroni’s multiple comparison test correction. (C) MCF7 cells were treated for 48 hours with treatments as above and real-time polymerase chain reaction (PCR) was performed for estrogen-responsive genes, progesterone receptor (PR) and stromal cell-derived factor 1 (SDF-1, also known as CXCL12). Each gene is normalized to 18S and shown relative to vehicle. *P < 0.05, ***P < 0.001, Student’s t test. MCF7 cells were treated with vehicle control, (D) 1 μM bicalutamide, 10 nM 5-alpha-dihydrotestosterone (DHT) and DHT + bicalutamide or (E) with 10 nM E2 and E2 + bicalutamide. **P < 0.01, ***P < 0.001 for DHT versus DHT + bicalutamide, or E2 versus E2 + bicalutamide, ANOVA with Bonferroni’s multiple comparison test correction. (F) MCF7 cells treated for 48 hours with vehicle, 1 μM bicalutamide, 10 nM E2 and E2 + bicalutamide and real-time PCR performed for PR and SDF-1. *P < 0.05, ***P < 0.001, Student’s t test. Error bars represent SEM, Student’s t test (all analyses).

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