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Figure 3 | Breast Cancer Research

Figure 3

From: Role of the androgen receptor in breast cancer and preclinical analysis of enzalutamide

Figure 3

Enzalutamide inhibits androgen-stimulated growth of MCF7 tumors in vivo . MCF7-TGL cells stably expressing luciferase were implanted orthotopically in the mammary gland of NOD/SCID ovariectomized female mice with a 5-alpha-dihydrotestosterone (DHT) pellet implanted subcutaneously. Mice were matched into two groups based on tumor volume (day -2) and treatment with either control chow (DHT) or chow containing 50 mg/kg enzalutamide (DHT + Enza) begun (day 0, indicated by arrow), and the tumor burden was measured by whole-body luminescence. (A) Mean total flux of all mice in each of the treatment groups. Error bar represents standard error of the mean. *P < 0.05, Wilcoxon rank sum. (B) Total luminescent flux is shown for all individual mice at the day of matching (day -2) and at the final imaging day (day 19). *P < 0.05, Wilcoxon rank sum. (C) Mice were injected with bromodeoxyuridine (BrdU) 2 hours prior to sacrifice and BrdU immunohistochemistry was performed on formalin-fixed paraffin-embedded tumor sections and quantified. *P < 0.05, Student’s t test. Representative images of BrdU staining (400× magnification) and quantification. (D) Quantification of apoptotic cells as measured by terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) staining with representative images below (400× magnification). *P < 0.05, Student’s t test. (E) Quantification of nuclear AR staining and representative images (400× magnification). ***P < 0.001, Wilcoxon rank sum.

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