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Figure 3 | Breast Cancer Research

Figure 3

From: New insights into lineage restriction of mammary gland epithelium using parity-identified mammary epithelial cells

Figure 3

Parity-identified mammary epithelial cells (PI-MECs) in involuted mammary glands are luminal. (A) Epithelial cells isolated from involuted mammary glands were labeled with fluorescent antibodies against CD24 and α6-integrin (CD49f) to distinguish the luminal (blue gate) and basal (red gate) population. (B) Yellow fluorescent protein-negative (YFPneg) cells and YFPpos cells (PI-MECs) are shown separately on density/contour plots. (C) The luminal and basal populations are plotted in separate histograms to quantify the proportion of YFP-positive cells in each population (D); error bars indicate standard deviation for 4 individual mice. (E) Immunofluorescent probing of involuted mammary epithelium for YFP (PI-MECs, green), the luminal marker cytokeratin-8 (CK8) (blue), and basal marker smooth muscle actin (SMA) (red). Nuclei are labeled by 4′,6-diamidino-2-phenylindole (DAPI) (grey). (F) Omission of the YFP signal from the image shown in (E) shows the mutual exclusive labeling of luminal cells by CK8 (blue) and basal cells by SMA (red). Bar is 10 μm.

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