Figure 7
From: GPR30 as an initiator of tamoxifen resistance in hormone-dependent breast cancer
Synergistic effects of GPR30 antagonist G15 and Tam on apoptosis. Cells were treated with ethanol (control), 1 μM Tam, 10 nM G15 alone or 10 nM G15 plus 1 μM Tam for 48 hours using Annexin V-FITC flow cytometry. Scattergrams show numbers of MCF-7 (A) and TAM-R (B) cells in early-phase apoptosis (EA) using the indicated treatments. (C) Histograms show percentages of MCF-7 (A) and TAM-R (B) cells in early-phase apoptosis 48 hours after using the indicated treatments. Data show means ± SD from three independent experiments. * P<0.05, versus control; ** P<0.05 versus Tam treatment. GPR30, G-protein coupled receptor 30; Tam, 4-hydroxytamoxifen; TAM-R, tamoxifen resistant.