Skip to main content
Figure 3 | Breast Cancer Research

Figure 3

From: Direct repression of MYB by ZEB1 suppresses proliferation and epithelial gene expression during epithelial-to-mesenchymal transition of breast cancer cells

Figure 3

MET/EMT cell states in the LA/ET breast cancer cell system. (A) Immunofluorescence for CDH1-repressor genes SNAI1, SNAI2, and ZEB1 (as indicated with green fluorescence (488) and nuclei stained with 4′,6-diamidino-2-phenylindole (DAPI), magnification 600×, scale bar, 50 μm; and (B) their expression along with other EMT-related genes, and MYB. Fold gene expression (for all QRT-PCR in this article, this is first corrected to housekeeping ribosomal protein L32; MYB, SNAI1, SNAI2, and ZEB1 are QRT-PCR data; all others are MT-PCR first corrected to the housekeeping genes L32, GUSB, and NONO) is shown, relative to PMC42-LA, which has been set to 1. Data shown are an average of three independent biologic replicates, error bars represent SEM. Student paired t test was used to determine significance; P < 0.05.

Back to article page

Breast Cancer Research

ISSN: 1465-542X

Contact us