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Figure 3 | Breast Cancer Research

Figure 3

From: Ltbp1Lis focally induced in embryonic mammary mesenchyme, demarcates the ductal luminal lineage and is upregulated during involution

Figure 3

Latent TGFβ binding protein (Ltbp)1L-LacZ is an early marker of mammary mesenchyme. (A) The 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal)-stained whole mount of embryonic day 14.5 female embryos. Ltbp1L-LacZ is expressed in a halo surrounding all mammary buds (white arrows). Expression is present but at lower levels in male embryos of the same stage (B, white arrows), coincident with the onset of sexual dimorphism. Localization of Ltbp1L-LacZ expression (blue stain) with immunohistochemical detection (brown stain) of epithelial markers p63 (C) and K14 (D), as well as mammary mesenchymal markers estrogen receptor (E), androgen receptor (F), and lymphoid enhancer-binding factor 1 (Lef1) (G). H = hindlimb, F = forelimb. Scale bars represent distance in microns.

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