Figure 10From: Ltbp1Lis focally induced in embryonic mammary mesenchyme, demarcates the ductal luminal lineage and is upregulated during involutionModulation of latent TGFβ binding protein (Ltbp)1L-LacZ expression during involution. (A, C, E) The 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal)-stained whole mounts and (B, D, F) nuclear fast red (NFR)-stained sections of lactating (A, B), and involuting glands removed 24 h (C, D) and 72 h (E, F) after forced pup weaning. (G) Immunofluorescent detection of frozen mammary glands isolated from mice at involution day 5 using anti-LTBP1 (red) and counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue) to detect nuclei. (H) Elastic fibers (purple), identified by resorcin-fuchsin staining, on X-Gal-stained paraffin-embedded sections of mammary gland harvested from mice at involution day 21 shows periductal arrangement of fibrils encasing the permanent duct. Scale bars represent distance in microns.Back to article page