Figure 3
From: A mammosphere formation RNAi screen reveals that ATG4A promotes a breast cancer stem-like phenotype
ATG4A expression regulates mammosphere formation in breast cancer cell lines. (A) Viability of SUM-149 cells cultured under adherent conditions with ATG4A inhibition or overexpression. (B) Number of mammospheres formed fourteen days post seeding 2,500 SUM-149 cells in serum-free suspension conditions. (C) Average size of SUM-149 mammospheres fourteen days post seeding and representative images of sphere numbers and sizes. Scale bars are 200 μm. (D) Relative number of colonies in soft agar fourteen days post seeding of SUM-149 cells. (E) Relative number of mammospheres at fourteen days post seeding of basal MDA-MB-231 or luminal MCF-7 cells with inhibited (shATG4A) or increased ATG4A expression (ATG4A-OE). CTRL, control.