Figure 2
From: Endoplasmic reticulum stress induces PRNP prion protein gene expression in breast cancer
PrP levels are significantly higher in high BiP-expressing breast cancer tumors. Representative micrographs of breast tissue cores immunostained for BiP (C50B12) (A) or PrP (3F4) (B), adsorption control for BiP (C) and PrP (D). E shows staining of PrP and BiP in normal versus tumor tissue cores. Mean intensity score (mean ± SEM) of PrP (F) or BiP (G) immunostaining correlated with tumor grade. H. Comparison of the mean intensity score (mean ± SEM) of PrP immunostaining in breast tissue cores showing Low (0 to 1) or High BiP (2 to 3) immunostaining scores. Correlation between averaged estrogen (I) and progesterone (J) receptor levels in cores (ERS/PR positive = 1 and ERS/PR negative = 0) correlated with PrP scores. Correlations were done with Spearman's rank correlation in InStat. ERS versus PrP: r = -0.2036, p = 0.0025, PR vs PrP: r = -0.2277, P = 0.0007, ERS vs BiP: r = -0.08819, P = 0.2108, and PR vs BiP r = -0.04398, p = 0.5353.