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Table 2 Comparison between CK19 RT-qPCR, multiplex RT-qPCR and AdnaTest for the detection of CTC in breast cancer

From: Comparison of three molecular assays for the detection and molecular characterization of circulating tumor cells in breast cancer

Molecular assay

CK19RT-qPCR

Multiplex- RT-qPCR

Total

Early breast cancer, (number = 254)

AdnaTest

Positive

Negative

Positive

Negative

Total (%)

Positive

4

38

5

37

42 (16.5)

Negative

32

180

53

159

212 (83.5)

Total (%)

36 (14.2)

218 (85.8)

58 (22.8)

196 (77.2)

254

Kappa test

poor agreement: Kappa = -0.059 (P = 0.344)

poor agreement: Kapp = -0.114 (P = 0.065)

 

Concordance (%)

184 (72.4), Pa = 0.344

164 (64.6), Pa = 0.065

 

Verified metastasis, (number = 51)

AdnaTest

Positive

Negative

Positive

Negative

Total (%)

Positive

17

11

16

12

28 (54.9)

Negative

4

19

4

19

23 (45.1)

Total (%)

21 (41.2)

30 (58.8)

20 (39.2)

31 (60.8)

51

Kappa test

Moderate agreement: Kappa = 0.422 (P = 0.002),

Fair agreement: Kappa = 0.386 (P = 0.004)

 

Concordance (%)

36 (70.6), Pa = 0.002

35 (68.6), Pa = 0.004

 
  1. achi-square test, kappa test; 95% CI (0.504, 0.848). The same result was found when we used the chi-square test (Table 2).