Skip to main content
Figure 3 | Breast Cancer Research

Figure 3

From: Hormone-sensing cells require Wip1 for paracrine stimulation in normal and premalignant mammary epithelium

Figure 3

Steroid-receptor-positive cells require Wip1 to respond to low levels of prolactin. (A, B) Confocal immunofluorescence of mammary gland sections from virgin wild-type (WT, A) and Wip1-knockout (KO, B) mice detecting estrogen receptor-α (ER, green), phospho-STAT5 (P-STAT5, red), and cytokeratin-8 (CK8, blue). Right panels, enlarged sections of images shown in A and B. (C) Percentage of ER+ luminal cells in virgin WT (blue bar) and Wip1 KO mice (green bar). Values represent the mean proportions from six mice/group ± SD. *P < 0.05. (D, E) Confocal immunofluorescence of 7-day pregnant WT (D) and Wip1 KO (E) mammary sections detecting estrogen receptor-α (ER, green), P-STAT5 (P-STAT5, red), and cytokeratin-8 (CK8, blue). Inset is enlarged section of the same image. Arrows, ER-positive cells; arrowheads, alveolar cells. Images are representative of five animals. Scale bar, 10 μm.

Back to article page