Figure 2

Serum starvation and hypoxia increase cell migration in transwell assays. (A) Effect of the addition of serum on the migration of MDA-MB-231 cells in a transwell assay during 24 hours. Effect of exposure to hypoxia on migration of MDA-MB-231 cells in a transwell assay under (B) serum-depleted or (C) serum-supplemented conditions, where cells were seeded in the transwell assays and then put in the hypoxic chamber for 24 hours (black bars) or cells received a 24 hour preincubation under the hypoxic conditions indicated on the x-axis and seeded in the transwell assays under hypoxia (white bars). Results are from two representative experiments with three replicates each. Asterisks indicate statistical significance of knockdown compared to the corresponding negative control.