Figure 6

Forced expression of vascular endothelial (VE)-cadherin in Sh-N-cad18-4 cell line does not accelerate tumor growth in vivo. (a) Western blot analysis for VE-cadherin in Sh-N-cadherin (Sh-N-cad2), Sh-N-cad2-expressing VE-cadherin cell lines (N-cadVE-1 and N-cadVE-2), and Sh-Scr. (b) Vimentin and neural (N)-cadherin mRNA expression levels in Ep5ExTu clones. Diagrams in (c) and (d) depict the quantification of the relative amount of vimentin and N-cadherin mRNA as determined in three independent experiments. β-actin was used as loading control. (e) Sh-N-cadherin (Sh-N-cad2), Sh-N-cad2-expressing VE-cadherin cell lines (N-cadVE-1 and N-cadVE-2) or Sh-Scr cell lines were injected into BALB/c mice. The mean ± SD is given (***, P < 0.0001) (n = 12).