Figure 5
From: Interplay between neural-cadherin and vascular endothelial-cadherin in breast cancer progression
The efficient suppression of neural (N)-cadherin in Ep5ExTu cells inhibits tumor growth in vivo without affecting cell proliferation in vitro. (a) Twenty-four hours after seeding equal numbers of cells, cell proliferation was quantified by measuring bromodeoxyuridine (BrdU) incorporation. The mean ± SD is (**, P < 0.0010) (n = 12). (b) Sh-N-cadherin cell lines (Sh-N-cad1.1, Sh-N-cad1.2 and Sh-N-cad2) or Sh-Scr were injected subcutaneously into BALB/c mice. (**, P < 0.0075 and ***, P < 0.0001) (n = 10). (c) Sh-N-cadherin cell lines (Sh-N-cad1.2 and Sh-N-cad2), Sh-VE-cadherin cell line (Sh-VE-cad-2) or Sh-Scr cell line injected into BALB/c mice. The mean ± SD is indicated (*, P < 0.334; **, P < 0.0083; ***, P < 0.0001) (n = 8). (d) Immunofluorescence staining for epithelial (E)-cadherin and vimentin on tumor sections. Nuclear staining with DAPI is also shown. Bar, 100 μm.