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Figure 1 | Breast Cancer Research

Figure 1

From: ERβ1 represses basal-like breast cancer epithelial to mesenchymal transition by destabilizing EGFR

Figure 1

ERβ1 inhibits invasion and migration in breast cancer cells by regulating EMT. (A) Control (Lenti), ERα- and ERβ1-expressing MDA-MB-231 cells following incubation with EtOH or 17β-estradiol (E2) for 24 h (scale bars, 50 μm). (B) Control (Lenti) and ERβ1-expressing Hs578T cells (upper panel) and Hs578T cells that were transiently transfected with a siRNA targeting luciferase (Control) or a specific ERβ siRNA (siRNA 3#) (lower panel) were photographed (scale bars, 100 μM). (C) Control (Lenti), ERα- and ERβ1-expressing MDA-MB-231 cells were incubated with EtOH or E2 and assessed for invasion by using matrigel-coated Transwell chambers. The cells that were translocated to the lower surface of the filter were shown (left panel) (scale bars, 500 μm). The graph shows the mean (cell number per field) of three separate experiments with the standard error of the mean (SEM) and P-value (*) ≤0.05% indicated. (D) Control (Lenti) and ERβ1-expressing MDA-MB-231 cells were incubated with E2 for 24 h and assessed for migration using wound-healing assay. The bar graph shows the mean (cells migrated into the wound) of three separate experiments with SEM and P-value (*) ≤0.05% indicated. (E) E-cadherin protein levels in control (Lenti), ERα- or ERβ1-expressing MDA-MB-231 cells. (F) E-cadherin expression was analyzed by immunoblotting in MDA-MB-231 cells transfected with control or ERβ siRNA (3#) (upper panel) and qPCR in MDA-MB-231 cells transfected with control or three specific ERβ siRNAs (lower panel). The graph indicates the mean of three separate experiments with SEM and P-value (*) ≤0.05%. (G) E-cadherin was visualized by immunofluorescence in control (Lenti) and ERβ1-expressing cells (scale bars, 20 μm).

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