Figure 6

Effect of RAD001, letrozole, and tamoxifen, alone or combination, on the growth of MCF7-AROM1 and BT474-AROM3 xenografts. MCF7-AROM1 xenograft was grown in the presence of androstenedione. Once tumors reached 7 to 8 mm in diameter, animals were randomized to receive vehicle, 0.5 mg/kg (n = 6), 2 mg/kg (n = 6), or 10 mg/kg RAD001 (n = 6). Bars represent ± SEM. (B) MCF7-AROM1 xenografts were grown as described earlier. Once tumors reached 7 to 8 mm in diameter, animals were randomized to receive vehicle (n = 4), tamoxifen (n = 4), letrozole (n = 5), RAD001 (2 mg/kg) (n = 5), or a combination of agents (n = 5). (C) BT474-AROM3 grafts were grown as described earlier. Once tumors reached 7 to 8 mm in diameter, animals were randomized to receive vehicle (n = 8), tamoxifen (n = 7), letrozole (n = 8), RAD001 (2 mg/kg) (n = 8), or a combination of agents (n = 8). Tumors were measured twice weekly, and points represent mean change in tumor volumes over that of day 0. Bars represent ± SEM.