Figure 2

Perp colocalizes with desmosome proteins. (A) Schematic diagram of the desmosome complex, highlighting several of the major protein components: Dsg, desmoglein; Dsc, desmocollin; Pg, plakoglobin; Dp, desmoplakin; IF, intermediate filaments. The depiction here of Perp's position within the desmosome is speculative, as its direct interacting partners have yet to be defined. (B) Dual-label immunofluorescence of wild-type virgin mouse mammary gland sections stained for Perp and the desmosome components Dp or Pg. Nuclei are marked with DAPI staining, and composite images are shown in merged panels. (C) Left, Composite images of Perp (green) and Dp (red) immunofluorescence on cross sections of wild-type mouse mammary glands at the indicated stages of development. Insets are shown at high magnification. Arrows indicate areas where Perp and Dp colocalize. Center and right, Representative H&E-stained sections of wild-type mouse mammary glands at the same stages. Images are taken at ×100 and ×630 magnification, respectively.