Figure 5

Quantitative analysis of the migration and invasion potential of MDA-MB-231 cells. The analysis was performed after staining with crystal violet the transwell chamber inserts to detect the cells migrated to the lower side of the porous membranes in the absence (migration) or presence (invasion) of Matrigel™. White columns represent control cells, and gray columns represent D609-treated cells. (a) Percentage of area occupied by migrating and invading cells in the absence or presence of D609. (b) Representative membranes containing cells that migrated in the absence or presence of Matrigel™. Examples of migration (c) and invasion (d) of MDA-MB-231 cells that were first treated with D609 (50 μg/mL) for 24, 48, and 72 hours, were subsequently detached, and were finally seeded in the transwell chambers and allowed to migrate in the absence of the inhibitor. CTR, control.