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Figure 5 | Breast Cancer Research

Figure 5

From: Protein tyrosine phosphatase Meg2 dephosphorylates signal transducer and activator of transcription 3 and suppresses tumor growth in breast cancer

Figure 5

The correlation of the PTPMeg2 expression and the pSTAT3 level. (A-D) Over-expression of PTPMeg2 decreases the tumor growth of v-Src transformed fibroblasts. (A) v-Src activated pSTAT3 was decreased by Ad/PTPMeg2. A Western blot was used to show the indicated proteins. STAT3 and pSTAT3 were examined in separated membranes from the same lysates. (B-D) 5 × 105 v-Src/NIH3T3 cells infected with Ad/PTPMeg2 or Ad/GFP were used to inject s.c. into the right (Ad/PTPMeg2) and left (Ad/GFP) flanks of 6-week-old male nude mice (n = 5). Xenograft tumors (B), tumor weights (C) and tumor volumes (D) are shown. (E) Over-expression of PTPMeg2 decreases the STAT3+/+ cell proliferation. Mouse hepatic STAT3+/+ and STAT3+/+cells infected with the indicated adenovirus were used to examine the proliferation rate by an MTT assay. Results were averaged from three repeats. (F) A Western blot was used to show the indicated proteins. (G) A high level of pSTAT3 is correlated with a low expression of PTPMeg2 in the breast carcinoma. Representative images of immunohistochemical staining of PTPMeg2, pSTAT3 and STAT3 are shown. All the tumor tissues were derived from surgery sections. Peri-tumor (P) or tumor (T) tissues are marked with dotted lines. Scale bars, 50 μm.

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