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Figure 4 | Breast Cancer Research

Figure 4

From: Protein tyrosine phosphatase Meg2 dephosphorylates signal transducer and activator of transcription 3 and suppresses tumor growth in breast cancer

Figure 4

PTPMeg2 inhibits breast cancer cell proliferation and tumor growth in nude mice. (A) PTPMeg2 expression correlates with the pSTAT3 level in breast cancer cells. The levels of pSTAT3, STAT3 and PTPMeg2 are shown. (B) Depletion of PTPMeg2 results in an increased level of pSTAT3 and expression of downstream gene expression. An shRNA targeting PTPMeg2 was stably expressed in MCF7 cells (MCF7/shPTPMeg2). Two downstream proteins CyclinD1 and Bcl-xL were examined. (C) Silencing PTPMeg2 increases the MCF7 cell proliferation. MCF7 stable cell lines were examined by an MTT assay. (D-F) Depletion of PTPMeg2 increases growth of tumors from MCF7 cells in nude mice. 1 × 107 MCF7 cells stably expressing shPTPMeg2 were injected s.c. into the right flanks of 6-week-old female nude mice (n = 6). Tumor weights (D), xenograft tumors (E), tumor sizes (F) are shown. (G) Over-expression of PTPMeg2 inhibits pSTAT3 and expression of its targeted genes. MDA-MB-231 cells were infected with Ad/PTPMeg2 or Ad/GFP. (H) Over-expression of PTPMeg2 inhibits MDA-MB-231 cell proliferation. MDA-MB-231 cells were examined by an MTT assay. (I-K) Over-expression of PTPMeg2 represses tumor growth. Tumor formation was observed in nude mice injected with 2 × 106 MDA-MB-231cells infected with Ad/PTPMeg2 or Ad/GFP. Xenograft tumors (I), tumor weights (J) and tumor volumes (K) are shown. All the data were obtained from 6 Balb/c-nu nude mice. Tumor formation was examined in nude mice injected with 6 × 106 MDA-MB-231cells infected with pMSCV/PTPMeg2 or pMSCV/vector virus. Xenograft tumors (L), tumor weights (M) and tumor volumes (N) are shown.

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