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Figure 6 | Breast Cancer Research

Figure 6

From: Honokiol activates AMP-activated protein kinase in breast cancer cells via an LKB1-dependent pathway and inhibits breast carcinogenesis

Figure 6

Honokiol treatment inhibited breast tumor growth in nude mice. MDA-MB-231 cells-derived tumors were developed in nude mice and treated with vehicle or honokiol (HNK). (a) Tumor growth was monitored by measuring the tumor volume for 4 weeks (eight mice per group). (b) At the end of 6 weeks, tumors were collected, measured, weighed, and photographed. Honokiol treatment inhibited tumor size as compare with vehicle treatment. Average tumor weight and representative tumor images are shown here. (c) Tumor samples were subjected to immunohistochemical analysis by using LKB1, p-AMPK, and Ki67 antibodies. Honokiol (HNK) treatment decreased the expression of Ki-67, increased expression of LKB1 and pAMPK, as compared with vehicle treatment. Bar diagrams show quantitation of protein expression in tumors from vehicle- and honokiol-treated mice. Columns, mean (n = 8); bar, SD. *Significantly different (P < 0.005) compared with control. (d) Tumor lysates (from two different tumors from each set) were subjected to immunoblot analysis by using phospho-AMPK (p-AMPK), AMPK, phospho-ACC (p-ACC), ACC, phospho-pS6K, pS6K antibodies. Actin antibody was used as control. (e) A model of honokiol (HNK)-stimulated AMPK activation in breast cancer cells. Honokiol stimulation induces LKB1 translocation from the nucleus into cytosol and phosphorylates AMPK, leading to increased phosphorylation of ACC and decreased phosphorylation of pS6K and 4EBP1.

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