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Figure 5 | Breast Cancer Research

Figure 5

From: Evidence that GTP-binding domain but not catalytic domain of transglutaminase 2 is essential for epithelial-to-mesenchymal transition in mammary epithelial cells

Figure 5

Transglutaminase 2 requires a functional GTP-binding domain to activate NF-κB and Akt signaling. (A) Electrophoretic mobility shift assay for NF-κB activity in the nuclear extracts prepared from the indicated MCF-10A cells. (B) Immunoblot analysis for different epithelial and mesenchymal marker proteins in transglutaminase 2 wild-type (TG2-WT) cells before and after transfection with either control or p65-specific siRNA. (C) Immunoblot analysis showing the levels of total and pAKT (S473) and phosphorylated focal adhesion kinase (pFAK) (Y397) in control and TG2-transfected MCF10A sublines.

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Breast Cancer Research

ISSN: 1465-542X

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