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Figure 3 | Breast Cancer Research

Figure 3

From: Evidence that GTP-binding domain but not catalytic domain of transglutaminase 2 is essential for epithelial-to-mesenchymal transition in mammary epithelial cells

Figure 3

GTP-binding inactive transglutaminase 2 is unable to induce the epithelial-to-mesenchymal transition transcription repressors. (A) Real-time RT-PCR array showing changes in the expression of epithelial-to-mesenchymal transition-related genes in MCF10A sublines expressing wild-type (WT) or mutant constructs of transglutaminase 2 (TG2) relative to control lentiviral vector (Vec)-transfected MCF10A cells. The y-axis denotes the fold change in transcript levels, the blue bars denote the gene expression in TG2-WT cells, the red bars denote gene expression in TG2-C277S and the green bars denote gene expression in TG2-R580A cells. The expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), β-actin and 18S ribosomal RNA was used to normalize variable template loading. (B) RT-PCR analysis (RT) and (C) Western blot analysis (WB) were performed to validate the expression of transcription factors Snail1, Zeb1 and Twist1 in different MCF10A sublines.

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Breast Cancer Research

ISSN: 1465-542X

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