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Figure 4 | Breast Cancer Research

Figure 4

From: Regulation of breast cancer metastasis by Runx2 and estrogen signaling: the role of SNAI2

Figure 4

WNT and TGFβ signaling are critical for Runx2-mediated stimulation of SNAI2 expression. (A) The 530 'common' genes, which were regulated by both Runx2 and E2, were analyzed for enrichment for developmental and growth factor signaling pathways using the IPA™ software package. The bars and the upper scale indicate the log2-transformed significance values. The line graph and the bottom scale show magnitudes of enrichment represented as ratios. (B) MCF7/Rx2dox cells were treated for 24 hours with vehicle control (C), dox (D), E2 (E) or both (DE), along with either 10 μM ICG-001, a small molecule inhibitor of Wnt signaling (top), or blocking antibody against the type I TGFβ receptor (bottom). DMSO and a non-specific antibody were used as respective controls. SNAI2 expression was measured by RT-qPCR and corrected for GAPDH. **, P < 0.01; ***, P < 0.005. DMSO, dimethyl sulfoxide; dox, doxycycline; E2, estradiol; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HGF, hepatocyte growth factor; IGF, insulin-like growth factor, IPA, Ingenuity Pathway Analysis; RT-qPCR; reverse transcription - quantitative polymerase chain reaction; Runx2, runt related transcription factor 2; TGF, transforming growth factor; SNAI2, snail homolog 2; VEGF, vascular endothelial growth factor.

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