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Table 1 PCR and DHPLC conditions for the mutational analysis of E-cadherin

From: Low frequency of E-cadherinalterations in familial breast cancer

 

Annealing

Amplicon

DHPLC

 

temperature

length

temperature

Exon no

(°C)

(bp)

(°C)

1

70

285

66

2

55

377

62

3

60

352

58, 61

4

60

351

60

5

60

398

58

6

58

246

59

7

60

329

60, 62, 64

8

58

225

59, 62

9

55

252

59

10

55

309

58

11

60

243

61

12

60

326

59, 61

13

58

297

58

14

60

206

61

15

60

244

58

16

60

318

59