Activation of NF-κB in macrophages leads to a shift in macrophage populations in the lung. Flow cytometric analysis of dissociated lung cells from IKFM and control mice. Three treatment groups (all 2 g/L doxycycline (dox)): one week dox - mice were on dox for one week (control n = 4, IKFM n = 4); two days dox - mice were on dox for one week, PyVT R221A cells were injected via tail vein, dox treatment was stopped two days after injection and mice sacrificed (control n = 3, IKFM n = 4); two weeks dox - mice were injected with PyVT cells via tail vein, two days later mice were put on dox and two weeks after cell injection mice were sacrificed (control n = 3, IKFM n = 3). All cells were gated for CD45+ and then for F4/80+ cells. (a) Representative flow diagrams of one week dox treatment group. (b) Graphs and (c) table showing percentage of cell populations that were Gr1+/CD11b+ (black), Gr1-/CD11b+ (grey) and Gr1-/Cd11c+ (white; *P < 0.05, **P < 0.01, ***P < 0.001; n ≥ 3 for all groups).