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Figure 3 | Breast Cancer Research

Figure 3

From: Dietary fat increases solid tumor growth and metastasis of 4T1 murine mammary carcinoma cells and mortality in obesity-resistant BALB/c mice

Figure 3

Dietary fat stimulates angiogenesis and immune cell infiltration in 4T1 tumors and macrophage infiltration in the gonadal fat pad in BALB/c mice. Mice were fed, injected with 4T1 cells, and then killed as described in Figure 1c. (a) Tumor sections were stained with antibody raised against CD31, vascular endothelial growth factor (VEGF), CD45, or CD68, and then with 1,3 -diaminobenzidine (DAB), and counterstained with hematoxylin, except that the last one was stained with hematoxylin and eosin (H&E) to visualize lipid vacuoles. Representative images of the immuno-histochemical analysis and H&E staining are shown, n = 15. (b) The staining intensity of CD31 and VEGF was quantified. CD45+ and CD68+ cells and the number of lipid vacuoles were counted. Each bar represents the mean ± SEM (n = 15). (c) Gonadal fat-pad sections were stained with an F4/80 antibody, and F4/80+ cells were counted, n = 15. Each bar represents the mean ± SEM (n = 15).*Significantly different from the control group, P < 0.05.

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