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Figure 2 | Breast Cancer Research

Figure 2

From: Dietary fat increases solid tumor growth and metastasis of 4T1 murine mammary carcinoma cells and mortality in obesity-resistant BALB/c mice

Figure 2

Dietary fat stimulates cell-cycle progression in 4T1 tumors in BALB/c mice. Mice were fed, injected with 4T1 cells, and then killed as described in Figure 1c. (a) Tumor sections were stained with antibody raised against Ki67, cyclin-dependent kinase (CDK)2, CDK4, cyclin D1, or cyclin A, and then with 1,3 -diaminobenzidine (DAB) and counterstained with hematoxylin (n = 15). Representative images of the immunohistochemical analysis are shown. (b) The Ki67-, CDK2-, CDK4-, cyclin D1-, and cyclin A-positive cells were counted. Each bar represents the means ± SEM (n = 15). (c) Tumor lysates were analyzed with Western blotting with the indicated antibodies. Photographs of chemiluminescent detection of the blots are shown. The relative abundance of each band to its own β-actin was quantified, and the control levels were at 100%. The adjusted mean ± SEM is shown above each blot. *Significantly different from the control group, P < 0.05. PCNA, proliferating cell nuclear antigen.

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