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Figure 1 | Breast Cancer Research

Figure 1

From: Dietary fat increases solid tumor growth and metastasis of 4T1 murine mammary carcinoma cells and mortality in obesity-resistant BALB/c mice

Figure 1

A high-fat diet reduces the survival rate and increases solid tumor growth in BALB/c mice injected with 4T1 cells. (a) Four-week-old, female BALB/c mice were fed on a high-fat diet in which 45% of the kilocalories were provided as fats or a control diet in which 10% of kilocalories were provided as fats for 12 weeks. Twelve weeks after initiating feeding, 4T1 cells (5 × 104 cells suspended in 0.1 ml gelatinous protein (Matrigel) were injected into the inguinal mammary fat pads of the mice. The mice were fed continuously on the same diets. Survival rates were monitored after the 4T1 cell injection (0 day). (b) Four-week-old, female BALB/c mice were fed on a high-fat diet in which 60% of the kilocalories were provided as fats or the control diet (10% kcal fat) for 16 weeks. Sixteen weeks after the initiation of feeding, 4T1 cells were injected, and mice were continuously fed on the same diet. (c-f) Mice were fed on the diets and injected with 4T1 cells, as described in b, except that they were killed 25 days after the 4T1 cells injection. (c) Body weights of mice were measured every week. Each point of body weight represents the mean ± SEM (n = 30). (d) Energy intake was calculated on the basis of 16.12 kJ/g in the control diet and 21.93 kJ/g in the high-fat diet. Each point of energy intake represents the mean ± SEM (n = 6). *Significantly different from the control group, P < 0.05. (e) The tumors were excised from mice and weighed. (f) The tumor volume was measured by using calipers and calculated with the formula: 0.52 × long diameter × short diameter2. Each bar represents the mean ± SEM (n = 30). *Significantly different from the control group, P < 0.05.

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