EpCAM and Mucin 1 enrichment also enriched for cells expressing epithelial transcripts. Cells from SLN samples were separated by IMS and the resulting cell fractions were analyzed by RT-PCR. Examples from three different RT-PCR positive SLN samples are shown. In all graphs, the first fraction corresponds to the MOC31 (anti-EpCAM) enriched cell population, that is, all cells which expressed the surface antigen EpCAM. The next fraction is the MOC31 depleted cells, that is, cells which did not express EpCAM. These are followed by Mucin 1 expressing cells (BM-7 positive), Mucin 1-depleted cells, and finally the control fraction corresponding to the non-separated cell population of the SLN sample. Due to the low amounts of template, all IMS enriched fractions were only analyzed for two PCR targets, while depleted- and control fractions were analyzed using all targets. SBEM was analyzed separately, and was positive in all three samples (not shown). The reference gene YARS was used for normalization of RT-PCR values and fold change was calculated relative to a positive control included on all PCR plates.