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Table 5 Antibodies used during Western blot analysesa

From: Allele-specific regulation of FGFR2 expression is cell type-dependent and may increase breast cancer risk through a paracrine stimulus involving FGF10

Antigen targeted

Antibody (catalog number, manufacturer)

Clonality

Dilution

Origin

Primary antibodies

    

   FGFR2

Bek C-17 (sc-122, SZ)

Polyclonal

1 in 500

Rabbit

   α-tubulin

Anti-α-tubulin (T6199, SA)

Monoclonal

1 in 8,000

Mouse

   ERK1/2

p44/42 MAPK antibody (9102, CS)

Polyclonal

1 in 100

Rabbit

   Phosphorylated ERK1/2

Phospho-p44/42 MAPK antibody (4377, CS)

Monoclonal

1 in 200

Rabbit

   FRS2α

FRS2 H-91 (sc-8318, SZ)

Polyclonal

1 in 50

Rabbit

   Phosphorylated FRS2α

Phospho-FRS2-α antibody (3864, CS)

Polyclonal

1 in 50

Rabbit

Secondary antibodies

    

Mouse IgG

IRDye 680 anti-mouse IgG (926-32220, LC)

Polyclonal

1 in 7,500

Goat

Rabbit IgG

IRDye 800CW anti-rabbit IgG (926-32211, LC)

Polyclonal

1 in 5,000

Goat

  1. aFGFR2: fibroblast growth factor receptor 2 gene; ERK1/2: extracellular signal-regulated kinases 1 and 2; FRS2α: fibroblast growth factor receptor substrate 2α; IgG: immunoglobulin G; MAPK: mitogen-activated protein kinase. Manufacturer names are abbreviated as follows: SZ: Santa Cruz Biotechnology, Santa Cruz, CA, USA; SA: Sigma-Aldrich, Zwijndrecht, The Netherlands; CS: Cell Signaling Technology, Danvers, MA, USA; LC, LI-COR Biosciences, Cambridge, UK. For each antibody, the target, manufacturer, clonality, dilution used during Western blot analysis and origin are given.