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Table 3 Primers used to genotype four SNPs in FGFR2a

From: Allele-specific regulation of FGFR2 expression is cell type-dependent and may increase breast cancer risk through a paracrine stimulus involving FGF10

SNP

Primer

Primer sequence (5' to 3')

Product length

Annealing temperature

Further analysis

rs10736303

F

AGGGACAAATACTCCGCACA

405 bp

50°C

Sanger sequencing

 

R

AGCCATCCAGCATGTTTCTC

   

rs2981578

F

TGACTCTTCAAAGTTTGTTTGTTTT

295 bp

50°C

Restriction enzyme AciI (New England Biolabs, Ipswich, MA, USA)

 

R

GAGGAAAGGTTCCCCACACT

   

rs2981582

F

AGCTCAGCTTACCCCAGACA

215 bp

58°C

Restriction enzyme AciI (New England Biolabs, Ipswich, MA, USA)

 

R

CGTGAGCCAAGCCTCTACTT

   

rs7895676

F

CAGGTGCGGTGGCTCATGTC

345 bp

67°C

Sanger sequencing

 

R

GACTTCAATGGCGGGACTCC

   
  1. aFGFR2: fibroblast growth factor receptor 2 gene; F: forward primer; R: reverse primer; bp: base pair. For each SNP, the forward and reverse primer sequences, the length of the resulting PCR product and the annealing temperature used during PCR are shown. Also shown is the technique that was used on the resulting PCR fragment to genotype the SNPs.