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Table 2 Primer sequences for the six marker genes used for CTC detection

From: The prognostic significance of tumour cell detection in the peripheral blood versus the bone marrow in 733 early-stage breast cancer patients

Gene

Accession

Sequence

Probe (5'FAM-3'TAMRA)

p1B

[Genbank: L15203]

Sense: CTGAGGAGTACGTGGGCCTG Antisense: AGTCCACCCTGTCCTTGGC

CTGCAAACCAGTGTGCCGTGCC

PS2

[Genbank:X00474]

Sense: GAGGCCCAGACAGAGACGTG Antisense: CCCTGCAGAAGTGTCTAAAATTCA

CTGCTGTTTCGACGACACCGTTCG

CK19

[Genbank:NM002276]

Sense: CTACAGCCACTACTACACGAC Antisense: CAGAGCCTGTTCCGTCTCAAA

CACCATTGAGAACTCCAGGATTGTCCTGC

EGP2

[Genbank:M32306]

Sense: CAGTTGGTGCACAAAATACTGTCA Antisense: CCATTCATTTCTGCCTTCATCA

TTGCTCAAAGCTGGCTGCCAAATGTT

SBEM

[Genbank:AF414087]

Sense: CTCTTGGGGAGTTTTCCATCTTTCTG Antisense: CTTCATCATCAGCAGGACCAGTAG

CCCAGAATCCGACAACAGCTGCTCC

MmGl

[Genbank:AF015224]

Sense: TTCTTAACCAAACGGATGAAACTCT Antisense: GGTCTTGCAGAAAGTTAAAATAAATCAC

TGCTGTCATATATTAATTGCATAAACACCTCAACATTG

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Breast Cancer Research

ISSN: 1465-542X

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