Figure 1From: Recruitment of regulatory T cells is correlated with hypoxia-induced CXCR4 expression, and is associated with poor prognosis in basal-like breast cancersImmunohistochemistry for FOXP3, CXCL12, CXCR4 and CA9. Immunohistochemical staining for (A) FOXP3 positive tumour infiltrating Treg (x20), (B) Tumour cells negative for CXCL12 (x10), (C) Tumour cells showing strong positivity for CXCL12 (x10). (D, E) Double immunohistochemical staining for FOXP3 (brown, nuclear) and CXCR4 (red, cytoplasmic). (D) CXCR4 negative Treg (x40), (E) CXCR4 positive Treg (arrows) (x40), Inset (x100 oil immersion), (F) Tumour cells with positive CA9 staining.Back to article page