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Figure 3 | Breast Cancer Research

Figure 3

From: Prospective isolation and characterization of committed and multipotent progenitors from immortalized mouse mammary epithelial cells with morphogenic potential

Figure 3

Immunofluorescence (IF) staining of CDβ-derived progenitor clones showing appropriate basal or luminal orientation. (A to C) CDβ multipotent outgrowths. (D to F) Ductal-limited outgrowths. (G to I) Alveolar-limited outgrowths. (J to L) Adult primary mammary outgrowths (primary BALB/C). (A, D, G and J) Na+, K+, 2Cl- type I cotransporter (NKCC1) (red) and mouse smooth muscle actin (SMA) (green). (B, E, H and K) Cytokeratin 5 (CK5) (red) and CK8 (green). (C, F, I and L) p63 (green) and nuclear estrogen receptor (ER) (red). The nuclei were counterstained with 4', 6-diamidino-2-phenylindole and TO-PRO-3 iodide.

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