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Figure 3 | Breast Cancer Research

Figure 3

From: Breast cancer cell migration is regulated through junctional adhesion molecule-A-mediated activation of Rap1 GTPase

Figure 3

Rap1 or β1-integrin protein antagonism reduces breast cancer cell migration. (a) Western blot analysis of active Rap1 protein expression in MCF7 cells following treatment with the Rap1 pharmacological inhibitor GGTI-298 (10 μM). Migration of MCF7 cells in scratch-wound assays after pretreatment for 2 hours with Rap1 inhibitor (b), MAb 13 anti-β1-integrin inhibitory antibody (c), J104 anti-JAM-A inhibitory antibody (d), or a combination of all three antagonists (e). Error bars refer to standard deviation and represent triplicate values in a representative experiment. (f) Graphic representation of migration differences between control treatments and inhibitor/antibody treatments alone and in combination. Error bars refer to standard deviation in pooled triplicate experiments. JAM-A, junctional adhesion molecule-A.

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