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Figure 4 | Breast Cancer Research

Figure 4

From: α-Tocopheryloxyacetic acid: a novel chemotherapeutic that stimulates the antitumor immune response

Figure 4

α-TEA results in increased responsiveness to T cell receptor (TCR) stimulation and induced a tumor-specific cytotoxic response. BALB/c mice with established 4T1 mammary tumors (day 10) received oral α-TEA in their diet for 16 days (day 26 post-tumor injection). (A) Tumor-draining lymph node cells were isolated, pooled from three mice per group and restimulated in vitro with plate-bound anti-CD3 antibody for 48 hours. Interferon (IFN)-γ secretion was determined by enzyme-linked immunosorbent assay. Combined results from two independent experiments are shown. (B) Splenocytes were isolated from three mice per group, pooled and restimulated in vitro for 6 days with 4T1 tumor cell lysate and then incubated with 51Cr-labeled 4T1 or Renca cells for 6 hours. 51Cr release into the supernatant was measured.

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