α-TEA cooperates with chemotherapeutic agents to induce apoptosis of p53 mutant, triple-negative human breast cancer cells via activating p73

Table 2 Half-maximal effective concentration values for apoptosis

Cell line	α-TEA (μM)	DOXO (μM)	CDDP (μM)
MDA-MB-231	41.7	46.5	70.8
BT-20	45.4	25.7	64.0
MDA-MB-468	35.4	8.5	40.7

Cells were treated with different concentrations of α-TEA, doxorubicin (DOXO), and cisplatin (CDDP) for 24 hours. Apoptosis was determined by annexin V-FITC/PI staining/fluorescence-activated cell sorter analysis as described in Material and methods. The concentration that achieved 50% apoptosis (half-maximal effective concentration) was determined using commercially available software (Calcusyn; Biosoft, Manchester, UK).

ISSN: 1465-542X