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Figure 2 | Breast Cancer Research

Figure 2

From: Inhibition of breast cancer cell invasion by melatonin is mediated through regulation of the p38 mitogen-activated protein kinase signaling pathway

Figure 2

Elevated phosphorylation of ERK1/2 and p38 MAPK in MCF-7/CXCR4, MCF-7/6, and MCF-7/Her2.1 cells. (a) Western blot analysis of Her2/neu receptor (top), phospho-ERK1/2 (p-ERK1/2) (middle), and phospho-p38 (p-p38) (bottom) in MCF-7/vec, MCF-7/Her2.1, MCF-7/AZ, MCF-7/6, MCF-7/pcDNA3, and MCF-7/CXCR4 breast cancer cells. The band intensity of Her2/neu, phospho-p38, and phospho-ERK1/2 was normalized to that of β-actin, total p38, and ERK1/2, respectively, and expressed as fold of control (MCF-7/vec, MCF-7/AZ, and MCF-7/pcDNA3, set as 1). (b) Western blot analysis of CXCR4 expression in MCF-7/pcDNA3 and MCF-7/CXCR4 cells. Levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are shown as loading control. The band intensity of CXCR4 was normalized to that of GAPDH and expressed as fold of control (MCF-7/pcDNA3, set as 1). ERK1/2, extracellular signal-regulated kinase 1/2; MAPK, mitogen-activated protein kinase.

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