Figure 7

Deficiency of Mena decreases macrophage recruitment to terminal end buds. A. Immunohistochemistry of terminal end buds of mammary glands from PyMT Mena WT, and Null mice. Blue = h ematoxilin and brown = F4/80+ macrophages (examples identified by black arrows). Scale bar = 50 μm. B. Quantification of macrophage number per surface unit of terminal end bud. N = 10 animals per genotype. P-values listed above bars. Error bars = SEM. C. Quantification of the number of macrophages around terminal end buds and the number of macrophages in surrounding stroma in 200× field in both Mena WT (white bars) and Mena Null (black bars) animals. N = 5 animals per genotype. P-values listed above bars. Error bars = SEM.